Proper aseptic technique and sterilization of all surgical materials are essential for generating high‑quality data and safeguarding animal welfare. All laboratories are strongly encouraged to review their current procedures to confirm that appropriate measures are in place to minimize both overt and subclinical infections, thereby promoting animal welfare and enhancing study reproducibility. Researchers are also encouraged to consult their facility veterinarian with any questions regarding expectations or best practices for aseptic technique. Please see additional resources below.

Accepted Sterilization Methods IACUC Guideline

• All materials that come into contact with the surgical site must be properly sterilized and handled using aseptic technique to prevent contamination.
• Ethanol is an intermediate-level disinfectant, NOT a sterilant, and is not effective or appropriate for use in sterilizing surgical materials.

ACLAM Position Statement on Rodent Surgery 

Example of importance of asepsis in a rat catheter model 

JOVE Article on Principles of Rodent Surgery for the New Surgeon