Rodent Breeding Colony Management

***All breeding animals in the same cage must be under the same approved protocol number that the breeding is intended for. This may require a "transfer" transaction slip being completed and processed before breeders are set up.*** 

  • Upon request, OAR will train research staff how to manage their breeding colonies:
    • Setting up breeding pairs
    • Weaning
    • ID system
    • Tail snips
  • Male rodents should never be combined for housing
    • Exception:  littermates that were always housed together and not used for breeding may remain housed together
  • A completed standard white cage card is needed for each animal

White Cage Tag
  • Green breeder card follows the female if she is removed from cage

Green Breeder card
  • Set up breeding pairs in a clean cage
  • Male
    • Must have a completed standard white cage card
  • Female
    • Must have a completed standard white cage card
    • Must have a completed green breeder card
  • Harem breeding
    • One male per breeding cage
    • Up to three females per breeding cage
      • Each female
        • Must have a completed standard white cage card
        • Must have a completed green breeder card
        • Once the females show signs of pregnancy each must be separated into her own cage (by laboratory staff)
      • Male
        • Must have a completed standard white cage card
        • Male may be rotated among the females that were separated (set up in clean cage) for future breeding
  • Litters must be weaned at day 21 +/- 3 days (18-24 days)
    • Separate rodents by sex
    • The maximum number of rodents per cage may vary -- see OAR facility supervisor for further information
    • Each cage should have  a completed standard white cage card
  • Tail tissue (for genetic analysis) is collected at weaning age

 

The following conditions will generate charges to an investigator:

  • OAR weans litter on or after day 25 (unless there is one or more IACUC approved variance)
  • OAR weans current litter if not weaned prior to the birth of a new litter
  • Separation of females with litters in harem breeding cages if minimum space requirements are exceeded
  • Completing standard white cage cards per rodent per breeding cage 

 

OAR Rodent Breeding Colony Management - Fee for Service

  • OAR management of breeding colonies (must be approved by OAR) is per instructions from PI.

 

OAR’s ear punch identification system (The rodent is facing you).

  • Each cage of animals is given a number
  • Each cage of animals will start with ear punch #1
  • No animal is assigned an ID # higher than 5

OAR’s ear punch identification system