IACUC Policy: Rodent Tail Snipping for Genotyping

Policy: The IACUC has provided a set of guidance documents (Policies, Guidelines, and Informational Sheets) for use when planning animal procedures at the University of Iowa. An exception to a Policy must be described and justified in the Animal Protocol and approved by the full IACUC at a convened monthly meeting.

Purpose: The intent of this policy is to describe procedures required for tail tissue collection in rodents for genetic analysis. This policy is intended for use by research staff and Office of Animal Resources staff approved to perform this procedure on an Animal Protocol. This policy is approved by the University of Iowa Institutional Animal Care and Use Committee (IACUC).

Procedures:

  • Mice and Rats ≤ 24 days old:  no anesthesia or analgesia required
    • Note: Higher DNA yield has been reported from tail snips at 18 days of age or younger, due to a lower percentage of ossified sample. For best results, we recommend collecting samples prior to day 18
  • Mice and rats >24 days old::
    • Tailing at this age is discouraged and should be avoided if possible
    • Procedures for tailing animals at this age must be described and justified in the Animal Protocol 
    • Use of a systemic analgesic given prior to tail snipping is required unless scientifically justified

Tail snipping procedure:

  • Gently, but securely, restrain animal (manual or mechanical)
  • Snip tail with sanitized sharp scissors or disposable blade
  • DO NOT remove more than 5mm of tail
  • Place tail tip into a tissue collection tube
  • Check for bleeding, if bleeding occurs:
    • Apply pressure to the cut portion of the tail with gauze until bleeding has stopped
    • If continuous pressure does not stop the bleeding, utilize a chemical cautery agent (e.g. silver nitrate or Kwik Stop®)
  • Return animal to its cage
  • Clean off biologic material (e.g. blood or fur) from scissors and sanitize after each snipping

References:

  1. Bonaparte, Dolores, Paolo Cinelli, Eleni Douni, Yanne Herault, Alex Maas, Pirjo Pakarinen, Matti Poutanen, Mirentxu S. Lafuente, and Ferdinando Scavizzi. "FELASA Guidelines for the Refinement of Methods for Genotyping Genetically-modified Rodents: A Report of the Federation of European Laboratory Animal Science Associations Working Group." FELASA Guidelines for the Refinement of Methods for Genotyping Genetically-modified Rodents: A Report of the Federation of European Laboratory Animal Science Associations Working Group 47.3 (2013): 134-45.
  2. Hankenson, F CLaire, Laura M. Garzel, David D. Fischer, Bonnie Nolan, and Kurt D. Hankenson. "Evaluation of Tail Biopsy Collection in Laboratory Mice (Mus Musculus): Vertebral Ossification, DNA Quantity, and Acute Behavioral Responses." Journal of the America Association for Laboratory Animal Science 47.6 (2008): 10-18.
  3. Jones, Carissa P., Scott Carver, and Lon V. Kendall. "Evaluation of Common Anesthetic and Analgesic Techniques for Tail Biopsy in Mice." Journal of the America Association for Laboratory Animal Science 51.6 (2012): 808-14.

 

Policy last updated/reviewed by Committee 4/14/15