Printer-friendly versionRecommended Volumes for Administered Substances (Informational Sheet)
Informational Sheet: The Office of Animal Resources has provided a set of guidance documents (Policies, Guidelines, and Informational Sheets) for use when planning animal procedures at the University of Iowa. This Informational Sheet provides the current guidance on recommended testing of research biologics for pathogens.
Purpose:
This document provides information about administration volumes and needle sizes for commonly employed routes of fluid administration in various animal species. The volume limits are consensus figures based on published literature and are set up to maintain animal welfare and prevent potential complications, such as muscle damage with high volume intramuscular injection, or aspiration & pulmonary injury with high volume oral administration.
Procedures:
- Give injections at a constant flow rate
- No resistance should be encountered during injection
- Do not apply overt pressure on the syringe’s plunger
- See below for recommended volume and needle size
- Compare the weight in the table to the weight of the animal being used in the procedure and alter dose accordingly
- Max limit with oil-based vehicles should be half the listed max limit and may require a larger gavage needle
- Always use the smallest needle size (largest gauge) that is applicable to the procedure
Precautions:
- Do not inject into inflamed or damaged tissue unless medically indicated
- Injecting relatively large volumes of solutions that are below body temperature may lead to hypothermia
- Inject separate drugs/compounds at different sites to avoid cross reaction of chemicals
- Limit subcutaneous administration to 2-3 sites per day
- Limit intramuscular administration to 2 sites at one time
- Exceptions apply when medically indicated or scientifically justified, consult with Office of Animal Resources (OAR) veterinary staff for guidance
Recommended Maximum Volumes and Needle Sizes per Route, per Site, and per Species
|
Species & weight |
Per os (oral) |
Subcutaneous |
Intramuscular |
Intraperitoneal |
Intravenous |
Rat200g |
4 ml 16 G |
4 ml 20 G |
21 G |
4 ml 21 G |
2 ml 23 G |
Mouse25g |
0.25 ml 18 G |
0.5 ml 20 G |
0.05 ml 23 G |
0.25 ml 21 G |
0.125 ml 25 G |
Hamster100g |
4 ml 18 G |
2 ml 20 G |
21 G |
2 ml 21 G |
1 ml, 25 G |
Guinea pig200g |
4 ml |
2 ml 20 G |
21 G |
2 ml 21 G |
1 ml 23 G |
Rabbit4kg |
80 ml |
20 ml 20 G |
0.5-1 ml 20 G |
40 ml 20 G |
20 ml 21 G |
Cat5kg |
100 ml |
100 ml 20 G |
1 ml 20 G |
100 ml 20 G |
25 ml 21 G |
Dog10kg |
200 ml |
50 ml 20 G |
5 ml 20 G |
200 ml 20 G |
50 ml 20 G |
Pig25kg |
250 ml |
62.5 ml 20 G |
2-5 ml 20 G |
250 ml 20 G |
250 ml 18 G |
Ferret1kg |
10 ml |
5 ml 21 G |
0.5 ml 23 G |
10 ml 21 G |
5 ml 21 G |
Goat/Sheep45kg |
450 ml |
450 ml 19 G |
5 ml 18 G |
450 ml 19 G |
450 ml 18 G |
Last Reviewed by the IACUC 6/10/2020