Guidelines: The IACUC has provided a set of guidance documents (Policies, Guidelines, and Informational Sheets) for use when planning animal procedures at the University of Iowa. An exception to a Guideline must be described and justified in the Animal Protocol and approved during the normal review process.
The purpose of these guidelines is to provide direction for personnel conducting non-survival surgery on research animals at the University of Iowa. These guidelines are intended for use by properly trained personnel listed on an IACUC-approved Animal Protocol who will be performing approved surgical procedures or assisting with those procedures. Deviation from these guidelines must be described and justified in an IACUC-approved Animal Protocol.
Instrument and Equipment Preparation
- All instruments should be visibly clean at the beginning of surgery
- Expired surgical materials such as suture or implants may be used
- All drugs (anesthetics, analgesics etc.) and any fluids administered must not be expired
- Allocate a clean uncluttered work area away from laboratory traffic, ventilation ducts and open windows
- Dedicate the area solely to surgical procedure(s) when in use
- Clean the work surface of visible dirt or debris
- Apply a clean drape over the working surface where the surgery will be performed
- Anesthetize the animal in accordance with the approved Animal Protocol
- For prolonged procedures, apply ophthalmic ointment to both eyes to prevent corneal desiccation
- Remove hair from the surgical site using one of the following:
- Clipper blade
- Depilatory cream
- Fur plucking
- Remove loose hair and visible dirt/debris from the surgical site
- Wipe the surgical site clean using alcohol, surgical scrub or warm water
- Avoid excessive wetting of non-surgical areas of the animal with alcohol or disinfectant as this can exacerbate hypothermia
- Record the following information in addition to the anesthesia record requirements Note: the anesthesia record may be combined with the surgical record; click here for the template.
- Time of euthanasia
Last Reviewed 1/11/17